Biotin-Streptavidin-Binding & Alternatives

Immobilisation of Biomolecules and coated Microplates

Immobilization of Biomolecules and Streptavidin Microplates

Streptavidin is a tetrameric protein (4 x 13 kDa), which binds very tightly to the small molecule Biotin. The binding constant for this interaction is very high (Ka ~ 1013 M-1) and turns the Streptavidin/Biotin system into a basis of many biochemical applications. Streptavidin-coated surfaces are characterised by very low nonspecific bindings.

Streptavidin coating of solid phases offers a universal immobilization principle for the detection and analysis of proteins, peptides, PCR-fragments, haptens etc.

Why use streptavidin-coated microplates and Polystrept R with lateral flow test stripes?

  • Convenient method for a uniform and stable immobilization of biotinylated molecules
  • Immobilization of Biomolecules on solid phases in a directed manner, while avoiding or minimizing denaturation
  • Ready-to-use, no washing or blocking steps required
  • High signal-to-noise ratio
  • Low nonspecific binding
  • Long shelf life
  • Lower reagent consumption (see table below) and especially enormous cost savings with lateral flow test stripes

Overview of BioTeZ‘s Streptavidin-Products:

  • sundry microplates with SC-coating for many kinds of Immunoassays
  • SC-coating kit components, for self coating of plates and other materials
  • sundry microplates with MC-coating for special assays with maximum biotin binding
  • MC-coating kit components with maximum biotin binding, for self coating of plates and other materials
  • Polystrept R, especially for cost reduction and Improvement of lateral flow test stripes
  • Various customized services
  • NEW:  TA-PAS Labelling System (fast 2-component protein ligand interaction system as an alternative or in combination to biotin-streptavidin)

Dear Customer, BioTeZ offers various Biotin-Streptavidin-Systems, components, conjugates and modules for pre-coatings, with and without blockings or stabilizers, for do it yourself or as customer service etc.

Save time instead doing long search, please write us an email and tell us what you are looking for.

Our streptavidin-coated products are ready-to-use. Additional washing or blocking steps are not necessary.

A basic prerequisite for the use of streptavidin-coated surfaces involves the use of components in biotinylated form. The biotinylation of these molecules is very simple and is a standard technique in many labs. Please contact us for our labelling service.

In the last few years we have focused our research on streptavidin modification and conjugation. We have innovative streptavidin coatings on many different surfaces (glass, plastics, metals, microspheres etc.) of very high biotin binding capacity and excellent thermo- and chemostability (up to +90°C) to improve your applications.

We also provide a special coating solution of our new Polystreptavidin molecule called Polystrept R for manufacturing your own Streptavidin surfaces with a very high biotin binding capacity.

Immobilizations to separate a target molecule we propose our services for custom immune affinity columns with activated Perl cellulose (for big-scale applications also).

Streptavidin Microplates:

BioTeZ streptavidin-coated microplates are manufactured by special procedures and patents guarantee a high homogeneity and reproducibility of the coated surface. Our coated microplates are suitable for immunoassays and also DNA hybridization assays.

Examples of material reduction for enzyme-linked immunosorbent assays when using

BioTeZ ‘Standard Capacity’ Streptavidin-coated microplates:

 Assay type  Biotinylated
materials
to be coated
Coating concentration required  Saving factor
 on streptavidin
 microplates
 for adsorptive
 coating on
MaxiSorp Strip1)
 Sandwich-ELISA  Capture antibody  0,6 – 0,75 µg/ml  2.5 µg/ml  70 – 75 %
 IEMA, indirect assay
(antigen: hapten)
 Hapten – immune
conjugate
 0.063 µg/ml  1.25 µg/ml  95%
 EIA, direct competitive assay
(antigen: hapten)
 Primary antibody  0.75 µg/ml  1.0 µg/ml  25%

1) Fisher Scientific, article number: 10554831

BioTeZ offers two kinds of streptavidin microplates arranged according to biotin binding capacity:

  • Standard Capacity (SC)’ for all standard immunoassays
  • Maximum Capacity (MC) with Polystrept R’ for an extraordinarily high biotin binding

Regular product range of BioTeZ streptavidin-coated microplates:

 Plate-Typ  Application  Coating-
Type
 Coating-
volume –
[µl/well]
 Biotin-
binding-
capacity
[pmol/well]
 Pack
Size
Order-No.  
 12 x 8-well strips
and frame, C well, polystyrene
strips, clear
 Colorimetric standard
immunoassays and sensitive
DNA hybridization assays
 SC  250  2  5 plates  F8PS-CL-SC
 12 x 8-well strips
and frame, C well, polystyrene
strips, clear
 DNA hybridization assays
with colorimetric
detection
 MC  200  > 50  5 plates  F8PS-CL-MC
 96 V-well,
polypropylene plate
 DNA hybridization assays
with colorimetric detection
or MALDI-TOF
 MC  20  20  –  on demand
 384 V-well,
polypropylene plate
 DNA hybridization assays
with colorimetric detection
or MALDI-TOF
 MC  20  24  –  on demand

General description of BioTeZ streptavidin-coated microplates:

Ready-to-use:              no additional washing or blocking steps required
Homogeneity:            < 3 % / plate (F8PS-CL-SC, F8PS-CL-MC) < 10 % / plate (V96PP-NT-MC, V384PP-NT-MC)
Leaching Stability:   no leaching up to 2 % SDS, 0.2 N NaOH and 0.2 % Tween 20
Package:                        each in a foil bag with desiccant

All other types of 96- and 384-well plates are coated inclusive of our special Streptavidin Coating Services.

Streptavidin Coating Services:

We offer individual service for streptavidin coating of various materials and surfaces.

We find out the best coating procedures for your application.

Our special coating procedures allow us to produce streptavidin layers with different biotin-binding capacities. We point out that especially our streptavidin layer with „Maximum Capacity“ has an extraordinarily high biotin binding capacity combined with a high chemo- and thermostability.

1. Customized coating service for any material or surface to suit your applications:

  • Materials like glass, metal, plastics
  • Inert plastics like Polycarbonate, Polypropylene, Polyethylene
  • Surfaces like membranes, slides, beads, tubes, foil

2. Customized coating service for any kind of microplates:

  • 96- and 384-well plates made of Polystyrene, Polycarbonate, Polypropylene or Polyethylene
  • Transparent, black, White
  • Well geometry (F, V, C-bottom etc.)

Polystrept R Coating Solution:

BioTeZ has designed a Polystreptavidin molecule called Polystrept R which has an extraordinary high biotin binding capacity. It has a very high chemo- and thermo stability, excellent adsorption capabilities and low unspecific bindings. BioTeZ offers Polystrept R as a ready-to-use coating solution for producing your own high capacity Streptavidin-coated materials. It is very suitable for coating Biochips, Plastics or Membranes and can be applied, for e.g., in Lateral Flow Tests.

If you are interested in our services, please use the order form or simply send us an E-mail to info@biotez.de

Alternative Detection System without Biotin:

TA-PAS Labelling System is a 2-component protein-ligand fast binding interaction system consisting of small synthetic TA-Label and PAS-Antibody. It can be flexibly combined with various target molecules and detection systems and is not influenced by Biotin.